Volume 194, number 12: A Complete Mutational Fitness Map of the Hepatitis C Virus Nonstructural 3 Protease: Relation to Recognition by Cytotoxic T Lymphocytes
14th Nov 2006, 00:59 GMT
The hepatitis C virus nonstructural (NS) 3/4A protease sequence is highly conserved for reasons not fully understood. We determined the protease activity in 181 NS3/4A gene products in which each protease residue was replaced by alanine or glycine. Unexpectedly, most (87%) protease residues could be replaced and protease activity would be retained. Using these data, we were able to identify a human leukocyte antigen A2–restricted epitope in which substitutions at 5 of 9 residues destroyed the protease. The NS3 protease shows an unexpectedly high plasticity, and it is therefore important to identify target sequences in which the appearance of mutations is restricted by viral fitness.
Volume 194, number 12: A Complete Mutational Fitness Map of the Hepatitis C Virus Nonstructural 3 Protease: Relation to Recognition by Cytotoxic T Lymphocytes related news:
- Volume 194, number 12: Equal Amounts of Intracellular and Virion-Enclosed Hepatitis C Virus RNA Are Associated with Peripheral-Blood Mononuclear Cells In Vivo — J Infect Dis Latest Issue
- Volume 194, number 12: Human Cytolytic T Cell Recognition of Yersinia pestis Virulence Proteins That Target Innate Immune Responses — J Infect Dis Latest Issue
- Fitness chain adds muscle with North Carolina expansion — bizjournals.com Sports Business:General headlines
- GolfFitnessProducts.net Announces Off-Season Golf Fitness Giveaway — PR Leap - Sports News Releases
- Tales From the Aussie Bargain Bin — IGN Complete
- Historical maps on Google Earth — kottke.org
- Health Tip: Living With Hepatitis C — MedicineNet Daily News
- Kaspersky Anti-Virus Update November 13, 2006 — MajorGeeks.com
- Allchin backs away from Vista anti-virus claims — The Register - Security: Anti-Virus
- Pick a 2008 Democrat, Pick a 2008 Republican - Map Colors all 50 States, Shows Electoral Outcome — GISUser.com - The complete GIS, LBS, and Geospatial Technology Resource
Latest news from J Infect Dis Latest Issue:
- Volume 194, number 12: Phase 1 Safety and Immunogenicity Evaluation of a Multiclade HIV-1 Candidate Vaccine Delivered by a Replication-Defective Recombinant Adenovirus Vector
- Volume 194, number 12: Phase 1 Safety and Immunogenicity Evaluation of a Multiclade HIV-1 DNA Candidate Vaccine
- Volume 194, number 12: Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification
- Volume 194, number 12: Cyclosporin A Provides No Sustained Immunologic Benefit to Persons with Chronic HIV-1 Infection Starting Suppressive Antiretroviral Therapy: Results of a Randomized, Controlled Trial of the AIDS Clinical Trials Group A5138
- Volume 194, number 12: Evaluation of Purified Taenia solium Glycoproteins and Recombinant Antigens in the Serologic Detection of Human and Swine Cysticercosis
- Volume 194, number 12: Phase 1 Clinical Trials of the National Institutes of Health Vaccine Research Center HIV/AIDS Candidate Vaccines
- Volume 194, number 12: Is There a Safe Age for Vaccinating Infants with Tetravalent Rhesus-Human Reassortant Rotavirus Vaccine?
- Volume 194, number 12: Reply to Gargiullo et al.
- Volume 194, number 12: Is Panton-Valentine Leukocidin the Major Virulence Determinant in Community-Associated Methicillin-Resistant Staphylococcus aureus Disease?
- Volume 194, number 12: Human Cytolytic T Cell Recognition of Yersinia pestis Virulence Proteins That Target Innate Immune Responses